Genome-Wide Analysis of WUSCHEL-Related Homeobox Gene Family in Sacred Lotus (Nelumbo nucifera).

WUSCHEL-related homeobox (WOX) is a plant-specific transcription factor (TF), which plays an essential role in the regulation of plant growth, development, and abiotic stress responses. However, little information is available on the specific roles of WOX TFs in sacred lotus (Nelumbo nucifera), which is a perennial aquatic plant with important edible, ornamental, and medicinal values. We identified 15 WOX TFs distributing on six chromosomes in the genome of N. nucifera. A total of 72 WOX genes from five species were divided into three clades and nine subclades based on the phylogenetic tree. NnWOXs in the same subclades had similar gene structures and conserved motifs. Cis-acting element analysis of the promoter regions of NnWOXs found many elements enriched in hormone induction, stress responses, and light responses, indicating their roles in growth and development. The Ka/Ks analysis showed that the WOX gene family had been intensely purified and selected in N. nucifera. The expression pattern analysis suggested that NnWOXs were involved in organ development and differentiation of N. nucifera. Furthermore, the protein-protein interaction analysis showed that NnWOXs might participate in the growth, development, and metabolic regulation of N. nucifera. Taken together, these findings laid a foundation for further analysis of NnWOX functions.

[Analysis of 21 autopsy cases of aortic dissection with disputes].

OBJECTIVE: To explore procedures and methods of forensic investigation for the death cases of aortic dissection with disputes. METHODS: Twenty-one death cases of aortic dissection with disputes from 2006 to 2011 were collected. All the data were analyzed to investigate the reasons and features of the disputes. RESULTS: The main causes of the disputes were clinical misdiagnosis or slight force. The pathologic type of De Bakey (I and II) or Stanford A were common in each case. For the cases dying of aortic dissection, it was significantly important to detect the rupture of entry and exit and observe the dissection of arterial wall. CONCLUSION: The main causes of clinical misdiagnosis are insufficient experiences, careless observation and curtness in medical treatment. The difficult points in these cases are confirmation of the relationships between injury and disease, the primary affection and complication.

[Application of multi-slice computed tomography in biomechanical analysis of traffic accidents].

OBJECTIVE: To study the application value of multi-slice computed tomography (MSCT) and 3D reconstruction in biomechanical analysis of traffic accidents in forensic medicine. METHODS: Based on a real case, the tomoscan images were obtained from a corpse by MSCT scanning. The 3D images were reconstructed. The biomechanic process of injury manners of impacting, rolling and crushing in traffic accidents was analyzed together with autopsy, vehicle inspection, etc. The MSCT results were compared with the autopsy results. RESULTS: Some characters in situ including the part of fracture on different site that suffered by force from different directions, trends of fracture line, and status of smash inner bones were obtained trough MSCT and 3D reconstruction. Some details like fracture were even better than those from autopsy. CONCLUSION: MSCT and 3D reconstruction have some advantages such as in situ reconstruction, easily controlled image and fully conserved evidence. It may be a supplementary method and have a directive function for the biomechanical analysis of traffic accidents.

[Judicial appraisal of 24 cases of medical tangles involving patient's death].

OBJECTIVE: To explore the cause of mistakes in medical tangles involving patient's death, and to analyze its key points in judicial appraisal. METHODS: Total 24 cases involving patient's death and multiple identifications were respectively analyzed and summarized based on common situations, distribution of departments, degree of responsibility and mistake analysis. RESULTS: It was showed that those medical tangles mostly originated from technical aspects, such as neglecting of latent symptoms, oversimplified management in clinical reception, poor cooperation between departments, and hesitation in confronting unexpected emergencies. In addition, some institutional mistakes, such as the performance of disclosure duty, opportunity of referral course, and deficiency of basal medical equipments, were the other aspects that caused these medical tangles. CONCLUSION: The results mentioned above could provide some clues for the judicial appraisal of the similar medical tangles, and be helpful for avoiding their occurrence in future.